The global prevalence of diabetes is projected to rise from 451 million in 2017 to 693 million by 2045, with significant mortality and economic costs driving a shift toward natural treatment methods. Therefore the interest of phenolic-rich plants, such as Phillyrea latifolia L. (mock privet), are gaining attention due to their potential to modulate carbohydrate metabolism. This study investigates the inhibitory effects of mock privet leaf (MPL) and fruit (MPF) aqueous extracts on sucrose transport across Caco-2 cell monolayers, providing an plant based alternative to synthetic drugs like acarbose.
The initial steps in developing a method to inhibit the transport of radiolabelled sucrose in the Caco-2 cell line involved quantifying the concentration of transported radiolabelled sucrose in the presence of MPL, MPF, and acarbose using a Liquid Scintillation Analyzer (LSC). 14C-labeled sucrose was employed for its ease of detection and high sensitivity. The study further investigated the mechanisms underlying sucrose transport inhibition, focusing on both transcellular and paracellular pathways. The MPL extract demonstrated more effective inhibition of 14C-sucrose transport than both acarbose and MPF, with an IC50 value of 0.32 mg/ml. In addition, the IC50 values for MPL and acarbose were found to be 1.01 mg/ml and 0.60 mg/ml, respectively. Notably, the exclusion of sodium from the transport buffer highlighted a significant inhibition of paracellular transport by both extracts and acarbose.
The aqueous extracts of mock privet leaves and fruits demonstrated promising acarbose-like activity, suggesting a potential role in reducing diabetes risk by interfering with sucrose hydrolysis and transport. These findings support the therapeutic potential of mock privet polyphenols as part of a comprehensive dietary and lifestyle strategy for diabetes management. ORCID NO: 0000-0002-5625-040X
Anahtar Kelimeler: Phillyrea latifolia L., Polyphenols, Caco-2, Sucrose transport, Type-2 Diabetes
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